The Oxygen Equilibrium of Cystine-treated Human Hemoglobin without Free Sulfhydryl Groups
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منابع مشابه
The Oxygen Equilibrium of Cystine-treated Human Hemoglobin without Free Sulfhydryl Groups.
In recent years there has been a good deal of speculation as to the possible role of sulfhydryl groups in determining the oxygen equilibrium of hemoglobin, especially the Bohr effect and the sigmoid character of the equilibrium curve. It is now known that human hemoglobin A contains six sulfhydryl groups (l-3), one in each of the LY chains and two in each of the /3 chains (3), but that only two...
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The rates at which the sulfhydryl groups on each chain of hemoglobin fi4 react with N-ethylmaleimide and iodoacetate have been measured. The sulfhydryl group at position 93 reacts with both alkylating agents at approximately the same rate as normal liganded hemoglobin. The sulfhydryl group at position 112, which is not available in normal hemoglobin, reacts in hemoglobin f14 and does so much mo...
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1. Addition of 2 moles of mersalyl, mercuric chloride, p-chloromercuribenzoate (PCMB), or methyl mercury hydroxide per mole of hemoglobin greatly reduces heme-heme interactions (n), yet these substances have quite different effects on the oxygen affinity (-log p(50)). Mersalyl and mercuric chloride at this concentration each increase the oxygen affinity, while PCMB and methyl mercury have littl...
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The theory that the alphabeta dimer is the functional unit of cooperativity in hemoglobin has been tested by determination of the oxygen equilibrium curve of stable deoxy dimers, obtained by the addition of 0.9 M MgCl(2) to human des-Arg 141alpha-hemoglobin. Cooperativity was absent in this medium, but was regained on transfer of the hemoglobin to a dilute phosphate buffer, where tetramers refo...
متن کاملThe oxygen equilibrium of fetal and adult human hemoglobin.
Although the fetal and adult hemoglobins of many species, including man, are essentially similar in molecular weight and in spectroscopic characteristics, they exhibit marked differences in other chemical and physical properties (l-6). Therefore, they may be assumed to represent distinct chemical entities. The present study was undertaken to determine whether the chemical difference is expresse...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)67882-2